FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745464
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34930.46856722792784095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26930.3612515158344333No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26190.3513248124657931No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25430.34112981981691937No Hit
CTGTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTC22660.30397175450457703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22440.3010205724220083No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21160.2838500584870631No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20770.27861841752250943No Hit
GATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCTTTGGATCTGGGACACATTTCA20330.27271605335737203No Hit
CTACTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT19520.26185033750791453No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAA18710.25098462165845703No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18350.24615541461425366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17330.2324726613223442No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16310.21878990803043474No Hit
CATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCC16180.21704602770891687No Hit
GTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCAAAGCCA16000.21463142418681522No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15340.2057778779391091No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14690.1970584763315197No Hit
CTCCTGATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTT14600.19585117457046886No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14400.1931682817681337No Hit
ACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA14280.19155854608673256No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT13970.187400062243113No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG13800.18511960336112812No Hit
GTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGC13600.1824367105587929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.17827822671517335No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12940.17358316431108678No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACGT12840.17224171790991918No Hit
GTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCTTTGGA12780.17143685006921863No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCA12220.1639247502226801No Hit
AACTACTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT12060.16177843598081193No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11880.15936383245871028No Hit
CCATAGATCAGGAGCGTCGGAGTTTTCCCTGGTTTCTGCTGATACCAGGC11790.15815653069765945No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11770.15788824141742594No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11760.15775409677730917No Hit
CCTTCCTCCCTGTCTGCGTCTTTCGGAGACAGAGTCACCATCACTTGCCG11700.1569492289366086No Hit
GTCTCCTTCCTCCCTGTCTGCGTCTTTCGGAGACAGAGTCACCATCACTT11530.1546687700546237No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11360.1523883111726388No Hit
GTGGATGCACCATAGATCAGGAGCGTCGGAGTTTTCCCTGGTTTCTGCTG11340.15212002189240525No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11320.15185173261217175No Hit
GGGCACTGTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGC11220.15051028621100415No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11160.1497054183703036No Hit
GAGTGAAATGTGTCCCAGATCCAAAGCCACTGAACCGAGATGGGACCCCT11130.14930298444995332No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11080.14863226124936954No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11020.14782739340866896No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC10950.14688838092785164No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10950.14688838092785164No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCCCCGCTCACTTT10920.14648594700750137No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10660.14299818636446562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10650.14286404172434886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10360.13897384716096284No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10180.13655924363886116No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10120.1357543757981606No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10010.13427878475687627No Hit
GCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCCCCGC9910.13293733835570865No Hit
TCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCAAAGCCACTGAACCGAGATGGGACC9710.13025444555337348No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9610.12891299915220586No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9600.12877885451208912No Hit
GTATCAACGCAGAGTACGGGGCAGAAGTCTCTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCT9570.12837642059173884No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9470.12703497419057125No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9410.1262301063498707No Hit
TCTCGGTTCAGTGGCTTTGGATCTGGGACACATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGC9290.12462037066846958No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9200.12341306890741874No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACGTCCAGATGACCCAGTCTCCTTCCTCC9130.1224740564266014No Hit
TTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAA9040.12126675466555058No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9000.12073017610508355No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8700.11670583690158076No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8480.11375465481901206No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8430.11308393161842825No Hit
CTGATGGTGAGAGTGAAATGTGTCCCAGATCCAAAGCCACTGAACCGAGA8360.11214491913761095No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8250.11066932809632658No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8220.11026689417597631No Hit
GTCCCATCTCGGTTCAGTGGCTTTGGATCTGGGACACATTTCACTCTCAC8160.10946202633527576No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8150.109327881695159No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACT8010.10744985673352436No Hit
GATCAGGAGCGTCGGAGTTTTCCCTGGTTTCTGCTGATACCAGGCTAAAT7960.10677913353294055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7870.10557183177188972No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7760.10409624073060537No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7720.10355966217013833No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG7670.10288893896955455No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7460.10007190152710259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)