FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745464
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32630.4377139607009862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24060.3227520041209233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22690.30437418842492725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21560.2892158440917335No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20430.27405749975853966No Hit
CTACTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT19700.2642649410300162No Hit
CTGTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTC19180.2572894197439447No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAA18190.24400910037238552No Hit
GATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCTTTGGATCTGGGACACATTTCA17830.23917989332818218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17350.2327409506025777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16680.22375325971475485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14950.20054623697455545No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14820.19880235665303758No Hit
CTCCTGATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTT14740.19772919953210347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14700.19719262097163648No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14640.1963877531309359No Hit
CATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCC14570.1954487406501186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14470.19410729424895098No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT14230.19088782288614875No Hit
ACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA14070.18874150864428063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13720.18404644624019403No Hit
GTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCAAAGCCA13520.18136355343785884No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13510.18122940879774208No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12970.17398559823143706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12850.17237586255003595No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12630.16942468046746723No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACGT12380.16607106446454825No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12380.16607106446454825No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12320.1652661966238477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12000.16097356814011138No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11930.1600345556592941No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC11920.15990041101917732No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11870.1592296878185935No Hit
GTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCTTTGGA11820.15855896461800972No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11770.15788824141742594No Hit
GTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGCAGGCCGC11770.15788824141742594No Hit
GTATCAACGCAGAGTACGGGGCAGAAGTCTCTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCT11700.1569492289366086No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11660.15641265037614158No Hit
CCTTCCTCCCTGTCTGCGTCTTTCGGAGACAGAGTCACCATCACTTGCCG11520.15453462541450694No Hit
GTCTCCTTCCTCCCTGTCTGCGTCTTTCGGAGACAGAGTCACCATCACTT11500.15426633613427343No Hit
AACTACTACAGTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT11270.15118100941158796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11160.1497054183703036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11070.14849811660925274No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10050.13481536331734328No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCCCCGCTCACTTT9950.1334739169161757No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9760.13092516875395727No Hit
GTGGATGCACCATAGATCAGGAGCGTCGGAGTTTTCCCTGGTTTCTGCTG9750.1307910241138405No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACGTCCAGATGACCCAGTCTCCTTCCTCC9630.1291812884324394No Hit
CCATAGATCAGGAGCGTCGGAGTTTTCCCTGGTTTCTGCTGATACCAGGC9330.1251569492289366No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9260.1242179367481193No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9180.12314477962718522No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCA9070.12166918858590085No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9040.12126675466555058No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8970.12032774218473327No Hit
TTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAA8950.12005945290449974No Hit
GGGCACTGTAGTAGTTTTGACAGTAATAAGAGGCAACATCTTCAGGCTGC8950.12005945290449974No Hit
TCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCAAAGCCACTGAACCGAGATGGGACC8890.11925458506379918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8800.11804728330274834No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8670.11630340298123049No Hit
GCCTGAAGATGTTGCCTCTTATTACTGTCAAAACTACTACAGTGCCCCGC8470.11362051017889528No Hit
GAGTGAAATGTGTCCCAGATCCAAAGCCACTGAACCGAGATGGGACCCCT8290.11120590665679361No Hit
GCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACGTCCAGATGACCCAGTCTCCTTC8190.10986446025562603No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8120.10892544777480873No Hit
GTCTCTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCC8080.10838886921434167No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8020.1075840013736411No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7910.10610841033235677No Hit
TCTCGGTTCAGTGGCTTTGGATCTGGGACACATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGC7880.10570597641200649No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCC7860.10543768713177297No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7860.10543768713177297No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7850.10530354249165622No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7740.10382795145037185No Hit
GGTATCAGCAGAAACCAGGGAAAACTCCGACGCTCCTGATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCAT7650.10262064968932101No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7550.10127920328815343No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7510.10074262472768637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA150.002385204468.78020529
TACGCAT252.6099625E-455.01307328
GCTCGTT400.00812578150.46211670
GTGGTAT2900.046.230111
ACGCATC306.409998E-445.8534729
GTATCAA28150.044.3288571
TATCAAC32800.037.926882
ATCAACG34550.035.9864923
TCAACGC34650.035.853754
CAACGCA35350.035.2290345
AACGCAG36250.034.7329446
CTACGCA400.00264538234.36699727
ATCGCCC400.002668121834.30706412
CGCAGAG38250.033.0065358
AGATCGA651.8556766E-532.9039766
GGGAACG1451.5559726E-632.4813670
AGAGTAC39500.032.22258411
CAGAGTA40150.031.70092210
GCAGAGT40350.031.6288159
CGCTTGA703.7863856E-430.5651969
Produced by FastQC (version 0.11.5)