FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841980
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC61730.733152806479964No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50420.5988265754530987No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT44590.5295850257725837No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC41450.49229197843179173No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA38290.4547613957576189No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34530.410104753082021No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32070.38088790707617753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31380.37269293807453857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30530.3625976864058529No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29930.3554716264044277No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC27790.33005534573267775No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26620.3161595287298986No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25800.30642058006128414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25560.303570156060714No Hit
GTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAA25230.2996508230599301No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGG24690.29323736905864745No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACAT23640.28076676405615336No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC23540.27957908738924914No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG22970.2728093303878952No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTT22850.2713841183876102No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22680.269365068053873No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT22280.2646143613862562No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT22060.262001472719067No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCA21910.26021995771871065No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21490.25523171571771297No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC19850.2357538183804841No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19430.23076557637948647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18580.22067032471080072No Hit
GTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGA18420.21877004204375403No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGT17960.21330672937599468No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17920.21283165870923298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17880.21235658804247132No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC17840.21188151737570965No Hit
AGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCCCTGATCTATG17320.2057055987078078No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA17020.20214256870709515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16450.19537281170574125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16300.1935912967053849No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15990.1899094990379819No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15710.18658400437065015No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGC15180.18028931803605786No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15060.17886410603577282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14930.17732012636879738No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14920.17720135870210693No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14870.17660752036865482No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAACTTAC14840.1762512173685836No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAAC14670.17423216703484642No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG14260.16936269270053922No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14170.16829378370032544No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14120.16769994536687333No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13730.16306800636594695No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13480.16009881469868642No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT13350.15855483503171097No Hit
GAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGC13340.15843606736502056No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13160.15629824936459297No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT12980.1541604313641654No Hit
GATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACAT12930.15356659303071332No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12900.15321029003064207No Hit
CCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAA12820.1522601486971187No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12510.14857835102971567No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12510.14857835102971567No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12410.14739067436281147No Hit
CCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCG12390.14715313902943064No Hit
CACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC12270.14572792702914558No Hit
ATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAATGGC11990.14240243236181382No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11870.14097722036152877No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11820.14038338202807668No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG11760.13967077602793415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.1375329580275066No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT11580.1375329580275066No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11450.13598897836053112No Hit
CTGCAAACTGGATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTT11220.13325732202665144No Hit
GTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCC11180.13278225135988977No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11170.13266348369319936No Hit
GCCCCTAGGTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGAT10810.12838784769234424No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10690.12696263569205918No Hit
ACTTATTACTGCCAACAGTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10660.12660633269198793No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10610.12601249435853584No Hit
CTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGC10350.12292453502458492No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10110.12007411102401482No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10090.11983657569063398No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10000.11876766669042019No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9880.11734245469013516No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9840.11686738402337349No Hit
GTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCA9810.11651108102330222No Hit
CAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCCC9660.11472956602294593No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9600.11401696002280338No Hit
ACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCAT9490.11271051568920876No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9480.11259174802251835No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9310.1105726976887812No Hit
GTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTC9290.11033516235540036No Hit
GGACCCCACTCTGCAAACTGGATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGG9260.10997885935532911No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9190.10914748568849617No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9140.10855364735504407No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9130.10843487968835364No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9090.10795980902159195No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9060.1076035060215207No Hit
CAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAG8940.10617829402123566No Hit
ACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGA8870.10534692035440271No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8850.10510938502102188No Hit
GGTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGA8790.10439677902087936No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8780.10427801135418893No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG8660.1028527993539039No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8530.10130881968692843No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8490.10083374902016674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.050.5065041
GGTATCA11250.045.1819951
CTTATAC2000.044.647751
TGGTATC3750.043.045012
GTATCAA29650.041.5839731
CGTATGC2050.040.53558341
TCGTATG2050.040.50890440
TACGGCG350.001376680439.25776319
TATGCCG2200.037.82843443
ACGAGAC2300.037.3597422
TATCAAC34700.035.433152
CGAGACG2450.035.07866723
CCCACGA2450.035.05157519
TTATACA2450.035.0453262
TATACAC2450.035.0453263
GTTCCTA400.002571648734.56618538
GCCGTCT2200.033.14759446
ATCTCGT2200.032.95952637
TCAACGC38300.032.550984
ATCAACG38050.032.4940643
Produced by FastQC (version 0.11.5)