FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841980
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC73100.8681916435069716No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46700.5546450034442624No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43490.5165205824366375No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA37030.439796669754626No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC33250.3949024917456472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31340.3722178674077769No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29070.3452576070690515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27660.32851136606570225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27210.32316682106463335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25310.3006009643934535No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25220.29953205539323974No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24420.2900306420580061No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC24050.2856362383904606No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22830.2711465830542293No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCA22120.2627140787192095No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC21530.2557067863844747No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG21410.25428157438418963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21400.25416280671749925No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGG20660.24537399938240814No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT20550.2440675550488135No Hit
GTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAA20310.24121713104824344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20250.24050452504810088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20180.23967315138126796No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19340.22969666737927266No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACAT19110.22696501104539302No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC18830.22363951637806123No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18630.22126416304425284No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT18110.21508824437635096No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTT18060.21449440604289888No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG17660.2097436993752821No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGT17310.20558683104111736No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.20487422504097486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17140.20356778070738024No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA16890.20059858904011973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16890.20059858904011973No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16100.19121594337157652No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15860.18836551937100643No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15640.1857526307038172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.18456495403691298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15510.18420865103684175No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15360.18242713603648542No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC15030.17850780303570155No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAACTTAC14980.17791396470224946No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14880.17672628803534526No Hit
GTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGA14820.17601368203520273No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAAC14720.17482600536829854No Hit
AGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCCCTGATCTATG14550.1728069550345614No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT14310.1699565310339913No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG14120.16769994536687333No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA14030.16663103636665955No Hit
CTGCAAACTGGATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTT13660.162236632699114No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG13420.15938620869854392No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA13360.1586736026984014No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12910.1533290576973325No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12780.151785078030357No Hit
GAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGC12640.15012233069669115No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12470.14810328036295398No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11980.14228366469512338No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11910.14145229102829046No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTCTGCAAACT11820.14038338202807668No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11820.14038338202807668No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11800.14014584669469582No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11730.1393144730278629No Hit
ACTTATTACTGCCAACAGTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11540.1370578873607449No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT11440.13587021069384073No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCGGGC11370.13503883702700778No Hit
GATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACAT11370.13503883702700778No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11340.1346825340269365No Hit
ATGCAGCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAATGGC11270.13385116036010355No Hit
GTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTC10910.12957552435924843No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10890.1293379890258676No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10760.12779400935889212No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT10660.12660633269198793No Hit
CACCATCAGTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC10210.12126178769091903No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10120.12019287869070525No Hit
CCTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCGACATAATTGCTAA10080.11971780802394356No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9810.11651108102330222No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9790.11627354568992138No Hit
GCCCCTAGGTCCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGAT9640.11449203068956508No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9570.11366065702273213No Hit
GTTGTCGGGCGAGTCAGGCCATTAGCAATTATGTCGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAGGTCC9560.1135418893560417No Hit
CCATCCTCACTGTCTGCATCTGTAGGAGACAGACTCACCATCAGTTGTCG9430.11199790968906626No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9150.1086724150217345No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8930.10605952635454523No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8780.10427801135418893No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8620.1023777286871422No Hit
CTGTAGGAGACAGACTCACCATCAGTTGTCGGGCGAGTCAGGCCATTAGC8610.10225896102045179No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8550.10154635502030929No Hit
GGTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGA8540.10142758735361886No Hit
CTGCTGCTCTGTTTCCCAGGTGTCAGATGTGACATCCAGATGACCCAGTC8450.10035867835340506No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8420.1000023753533338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3850.049.0623172
GTGGTAT4100.046.9279171
GGTATCA12350.046.4597241
GTATCAA33950.042.099861
CGACAAG350.001392214439.16824714
TATCAAC38500.037.287362
TCAACGC41550.034.4061624
AGTGACG400.002639726334.38250730
ATCAACG41650.034.3562163
CAACGCA42350.033.8210955
AACGCAG42900.033.4653786
GCTTGTC1305.456968E-1231.715021
ACGCAGA45550.031.5184367
CGCAGAG45900.031.3527478
CGTGCAA450.004333453731.07333257
CAGAGTA46850.030.79012910
AGAGTAC46700.030.74228511
AACGGCC450.00462487830.66273737
CCGTCTG450.00466832930.60418134
CGCGGTA500.00754851127.73742943
Produced by FastQC (version 0.11.5)