FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082126
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAACCTGTGAATGAGCAACAATAATAAAGAG62460.29998184547909207No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59670.28658208004702884No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51370.2467189785824681No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48480.23283893481950657No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC48150.23125401632754214No Hit
CTCCTGCACTGGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACAACACCCAGGCAAAGC46950.2254906763567623No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTT45330.21771016739620944No Hit
ATATATTACTGTGCGATAGCCACGAGACACGCTTTTGACATGCGGGGCCA43580.20930529660548883No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42780.2054630699583022No Hit
GTAATATATGGCCCAGTCGTCGGCTCTCAGGTTGTCCATTTGTAGATACA42150.2024373164736428No Hit
GCCTTGGGCTGACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATA40600.1949930023447188No Hit
ACTATAACCTGTGAATGAGCAACAATAATAAAGAGCCTCGTCTTCAGTCTGGAGCCCAGAGATTGTCAGGGAGGC40520.19460877968000015No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39680.19057444170045423No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38900.18682827071944733No Hit
GTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG38750.18610785322309986No Hit
GGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT38160.18327421107079975No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT35280.16944219514092806No Hit
GTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACAACA34070.16363082733705836No Hit
GGTTATAGTTATGTCTTCGGATCTGGGACCACGGTCACCGTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACT32690.1570029863706615No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31920.15330484322274446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.1456684177614611No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30300.14552433426219163No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29480.14158605194882537No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAAC29270.1405774674539389No Hit
GCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29220.14033732828848974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29020.1393767716266931No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27950.13423779348608106No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT27750.1332772368242844No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27690.13298906982574543No Hit
GCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA26560.12756192468659436No Hit
CTCCTCACTCAGGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGA26200.12583292269536042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26190.12578489486227057No Hit
GGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26180.12573686702918074No Hit
GGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACA26140.12554475569682141No Hit
TCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25180.12093408372019752No Hit
ACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAACCTGTGAATGAGCAACAATAATAAAGAGC24450.11742805190463979No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT24430.11733199623846012No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGC24180.1161313004112143No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23900.11478652108469901No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCTTGGGC23600.11334568609200404No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23330.11204893459857856No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGAC23170.11128048926914126No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22370.10743826262195467No Hit
CCCCTGAGGGCCGCTCAGTGACCTCATGAATCATGACTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAAGAGA22010.10570926063072071No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT21440.10297167414460029No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21430.10292364631151044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8350.048.6519932
GTGGTAT8450.048.4848251
GGTATCA26900.044.0272671
GTATCAA64100.038.188021
CTACGCT2950.037.35941328
TATCAAC72100.033.9499742
ACTACGC3300.033.39063327
TCAACGC77750.031.7928284
ATCAACG77700.031.7689783
CAACGCA79850.031.129165
AACGCAG81250.030.6351556
TACGCTG3600.030.61837829
AGTCGTC15300.029.69214615
CAGTCGT15250.029.56310714
TAATATA15600.029.351632
GTCGGCT15100.029.17654219
CGTCGGC15300.028.7944618
CGAGACT3950.028.75666223
GTAATAT15800.028.5449811
CGCAGAG87450.028.4515728
Produced by FastQC (version 0.11.5)