FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082126
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAACCTGTGAATGAGCAACAATAATAAAGAG56640.2720296466208097No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44720.2147804695777297No Hit
CTCCTGCACTGGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACAACACCCAGGCAAAGC44420.21333963458503472No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43940.2110342985967228No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43650.20964149143711763No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTT42220.20277351130527163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39830.19129485919680173No Hit
GCCTTGGGCTGACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATA39000.18730854905034564No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37170.17851945559490637No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36170.17371667228592313No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35400.17001852913800605No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT35310.16958627864019757No Hit
GTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG35240.16925008380856874No Hit
ACTATAACCTGTGAATGAGCAACAATAATAAAGAGCCTCGTCTTCAGTCTGGAGCCCAGAGATTGTCAGGGAGGC34720.16675263648789745No Hit
GGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT34510.16574405199301098No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33770.1621899923443634No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32760.15733918120229035No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31680.15215217522858848No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT31130.1495106444086477No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30920.14850205991376123No Hit
GTCGATCACCATCTCCTGCACTGGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACAACA29540.14187421894736438No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29380.14110577361792706No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAAC27450.13183640183158943No Hit
GGTTATAGTTATGTCTTCGGATCTGGGACCACGGTCACCGTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACT27380.13150020699996062No Hit
GTAATATATGGCCCAGTCGTCGGCTCTCAGGTTGTCCATTTGTAGATACA27260.13092387300288263No Hit
GCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26940.129386982344008No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGC25880.12429603203648579No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25840.12410392070412646No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25800.12391180937176711No Hit
GGATCCAGCAGTGATGTTGGAAAATATAACCTTGTCTCTTGGTACCAACA25630.12309533620923999No Hit
GGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25530.12261505787834166No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT24270.1165635509090228No Hit
CTCCTCACTCAGGACACAGGGTCTTGGGCCCAGTCTGCCCTGGCTCAGCCTGTCTCCGTGTCTGGGTCTCCTGGA24160.11603524474503464No Hit
GCCTGTCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA24120.11584313341267531No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23630.11348976959127353No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCTTGGGC22600.10854290278302081No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21960.10546912146527154No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21860.10498884313437323No Hit
ACCTAAGACGGTGACCGTGGTCCCAGATCCGAAGACATAACTATAACCTGTGAATGAGCAACAATAATAAAGAGC21420.10287561847842062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21250.10205914531589345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9000.052.3617131
CGTAACG200.007385599451.7043335
TGGTATC9400.049.747262
GGTATCA25550.042.5433771
GTATCAA68250.040.773841
TATCAAC75050.036.8810542
ATCAACG79300.034.8342743
TCAACGC79200.034.7664224
CAACGCA81450.033.7962845
AACGCAG83700.033.1330266
CGCAGAG90600.030.5717688
AGAGTAC91300.030.26218811
GCAGAGT92900.029.7409869
CAGAGTA92850.029.72003710
CCTTTGT18850.029.14480670
ACGCAGA95950.028.9029087
CGTTTAC500.007029358828.14723663
AATCGAG1000.00791627127.4689870
GAGTACG59300.026.62494912
AGTACGG58950.026.55331813
Produced by FastQC (version 0.11.5)