FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720471
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53010.3081133015319642No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA41900.2435379614070798No Hit
CTATAATAACTGGCCTCGATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT40180.23354069902950994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34980.20331641742290335No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34400.19994524755139725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34380.19982900031444878No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34380.19982900031444878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32940.19145919925415775No Hit
GTTATTATAGTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT30410.17675392378017415No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCACTATAATAACTGG30110.17501021522594684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28790.1673378975873467No Hit
ATAATAACTGGCCTCGATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT28030.1629205025833042No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATCGAGGCCAGTTATTATAGTG27460.15960745633027235No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAGTTCA26870.15617816284029198No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG26570.1544344542860647No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26300.15286511658726012No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAGTTCACT25490.1481571034908464No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT25310.14711087835831002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24650.14327471953900994No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23590.13711361598074015No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAACTTAGCCTGGTA22370.13002253452688248No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21410.12444266715335511No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21280.12368706011318994No Hit
GTTTATTACTGTCAGCACTATAATAACTGGCCTCGATTCACTTTCGGCCC20720.1204321374786323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.12031589024168382No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC19890.1156078771452701No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19240.11182984194444429No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18300.1063662218078654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18170.10561061476770024No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17400.10113509614518351No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC17330.1007282308158638No Hit
TCTATGGTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAGTTCACT17310.10061198357891532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64550.040.1018261
GGTATCA26350.039.399591
TATCAAC73200.035.4099772
ATCAACG77700.033.4034463
TCAACGC77800.033.3163264
TCGTATG2500.033.20441440
CAACGCA79050.032.702535
AACGCAG81100.031.960676
CGCAGAG87200.029.640018
ACGCAGA87600.029.6232367
AGAGTAC87400.029.37556311
GTGGTAT14100.029.013041
GCAGAGT90500.028.4832619
CAGAGTA92750.027.68112410
GTCGTAT1155.5410055E-827.0506239
CTCGTAT2500.026.26915739
CGTATGC3350.025.8375841
TATACAC5150.025.3654023
AGTACGG60800.025.26858913
GAGTACG61000.025.1857412
Produced by FastQC (version 0.11.5)