FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654140
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.11037392607087168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.040.563421
GGTATCA12750.039.3395351
GTATCAA29550.037.7843931
TGGTATC4800.036.5019042
TATCAAC33400.033.7375832
ATCAACG35700.031.6602463
TCAACGC36050.031.2575654
CAACGCA36600.030.8817165
CGACCGT450.00467365930.59603932
GTATAGC450.00471761930.537541
AACGCAG37650.029.8379766
TACCGTT500.00785808527.50903329
ACGCAGA41050.027.4377217
CGCAGAG41600.027.1575098
AGAGTAC43000.026.03373711
CAGAGTA43300.025.93266910
GCAGAGT44300.025.2697689
TCGTATG1401.3365934E-824.72630140
CGTATGC1502.7333954E-823.11528841
GAGTACG27300.022.51529912
Produced by FastQC (version 0.11.5)