FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654140
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15680.23970403889075734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.042.0948031
GTATCAA33200.040.8569151
GGTATCA12900.037.534961
TGGTATC5400.036.867292
TATCAAC37350.036.3924752
ATCAACG38450.035.151263
TCAACGC38350.035.0398864
CAACGCA39400.034.264375
AACGCAG40350.033.6274876
ACGCAGA42700.031.8570387
CGCAGAG43750.031.2491078
AGAGTAC44650.030.84944511
CGTCCGC450.004732505430.51787428
CAGAGTA45450.030.15566310
GCAGAGT45800.029.7007759
GAGTACT23700.026.74644912
GAGTACG28650.025.95242112
AGTACTT24150.025.68054413
AGTACGG28800.025.57930413
GTACTTT25050.024.90038314
Produced by FastQC (version 0.11.5)