FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714009
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.10406031296524275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.042.1364141
GTATCAA32700.038.5792661
GGTATCA13950.037.947411
GTACTAA601.3757617E-534.374441
TGGTATC5750.034.075532
TATCAAC37050.034.0497132
TCAACGC38000.033.198474
ATCAACG38050.033.1548463
CAACGCA38700.032.8644525
AACGCAG39450.032.3267866
ACGCAGA42300.030.30497
CGCAGAG42450.030.0337948
AGAGTAC44000.029.2100911
CGGGATT953.308778E-728.94283917
CAGAGTA44900.028.5480510
GCAGAGT45950.027.7461249
GCGTAGC500.00787179527.499551
CGCGCAT658.568754E-426.58339738
GAGTACG29300.025.45107312
TCGTATG1101.121718E-625.16706740
Produced by FastQC (version 0.11.5)