FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065331
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17200.16145216838710222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12750.11968111319392752No Hit
GTATTACTGTCAGCAGTATGGAAACTCACCCGGAACGTTCGGCCAAGGGA11510.10804153826369457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8050.040.2178341
GGTATCA18450.038.8287731
GTATCAA42600.038.241791
TGGTATC8500.038.05822
CCGTTCG601.3849762E-534.3432589
TATCAAC47100.034.3412632
TCAACGC50100.032.3064884
ATCAACG50300.032.2007183
CAACGCA51150.031.692635
AACGCAG52000.031.3712486
ACGCAGA55750.029.2610727
CGCAGAG55650.029.251948
AGAGTAC57050.028.59430111
CAGAGTA59250.027.70646310
GCAGAGT59250.027.5325729
GAGTACG40700.024.80815312
AGTACGG40200.024.60643613
CGTATAG700.001353243124.5816652
GAGTACT26300.023.63547512
TGTTAGA5350.023.51269570
Produced by FastQC (version 0.11.5)