FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759712
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGC54730.3110168027495408No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41680.23685694022658252No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGGGTGTTGTCGTAGGA37980.2158307723081959No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36130.20531768834900258No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTG35230.20020321507155714No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32520.18480296775836047No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29040.16502700441890492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28180.16013984106490153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27670.1572416395410158No Hit
GGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGC26490.1505359967994763No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGG25650.14576248840719389No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25570.14530786856030986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25400.14434180138568128No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC24990.1420118746704006No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAG24630.13996608535942245No Hit
ACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG24160.13729519375897872No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC24140.13718153879725775No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23720.13479478460111655No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT22740.1292256914767871No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22610.12848693422560056No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22210.12621383499118038No Hit
GAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT22010.12507728537397028No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC21760.12365659835245767No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21310.12109936171373498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20750.11791702278554674No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19980.11354130675928788No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19470.11064310523540215No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT19130.108710970886145No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19110.10859731592442401No Hit
GTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG18390.10450573730246768No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18340.10422159989816515No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17970.10211898310632649No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17620.10013002127620885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8800.049.2712171
TGGTATC8850.045.8534622
GGTATCA27600.043.513281
GTATCAA70400.039.9839861
TATCAAC76900.036.268362
ATCAACG82350.033.634393
TCAACGC82900.033.4346284
AACGCAG86750.032.054986
CGGATAG850.003989362631.603470
CAACGCA88800.031.2007645
CGCAGAG92350.030.1112028
AGAGTAC93250.029.857411
ACGCAGA93350.029.7150887
CAGAGTA95750.029.14954210
GCAGAGT98850.028.3743119
CTCGTAT4350.027.43645160
AGTACGG66300.026.66999813
CACTCGT4600.026.65345658
GAGTACG67900.026.0898912
CGTATAT4700.025.45395562
Produced by FastQC (version 0.11.5)