FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080707
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32550.1564372110056822No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACCC29580.1421632166374218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27240.13091703925636816No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21680.1041953528295911No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA21610.10385892871990145No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21290.10232098993274881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36500.042.140231
GTATCAA87350.039.9051361
GTGGTAT12250.039.3254971
TGGTATC12700.037.9110682
TATCAAC95650.036.5300642
TCAACGC103650.033.6991234
ATCAACG103700.033.677293
CAACGCA105350.033.2045675
AACGCAG108300.032.4578136
ACGCAGA116750.030.0792147
CGCAGAG119100.029.4857148
AGAGTAC119450.029.08249711
CAGAGTA120750.028.74165510
GCAGAGT123200.028.3651359
AGTACGG81400.026.02195213
GAGTACG82900.025.71366512
TATATCG1052.3714025E-522.8858455
GTACGGG92650.022.53417414
GAGTACT59600.022.4042412
AGTACTT59900.022.12277213
Produced by FastQC (version 0.11.5)