FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731746
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.12617323787668633No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21400.12357470437350514No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19450.11231439252638667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61550.040.2458951
GGTATCA25750.040.1388441
TATCAAC69050.035.7248272
ATCAACG73150.033.6282843
TCAACGC73300.033.5124634
CAACGCA75050.032.8687485
AACGCAG76700.032.25156
GTGGTAT11000.031.320461
ACGCAGA82450.029.9945057
CGCAGAG83950.029.458578
CAGAGTA87750.028.1828710
GCAGAGT88600.027.9902469
TGGTATC12850.027.3475132
AGAGTAC91700.026.89376611
TCGTATG1306.151822E-926.60257540
AGTACGG57300.026.3285813
GAGTACG58200.025.92143812
TCGGATA7250.025.19834129
TACGATC7350.024.51332343
CGGATAT7800.023.86896730
Produced by FastQC (version 0.11.5)