FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846759
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.14425271516207583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24830.13445176116645433No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22530.12199751023279162No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA19870.10759389828342517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.042.234931
GTATCAA65650.038.543321
TATCAAC74350.034.1711922
ATCAACG78600.032.367313
TCAACGC79100.032.0321464
CAACGCA81500.031.0888715
AACGCAG82450.030.730666
GTGGTAT11950.029.6732981
ACGCAGA90600.027.957177
CGCAGAG91550.027.5903878
TGGTATC12850.027.0584552
CAGAGTA95500.026.37714610
GCAGAGT95950.026.3610369
AGAGTAC100200.025.24292811
ACTCGAC2100.024.60322427
GAGTACG61500.023.94907612
AGTACGG61300.023.91493613
CTCGACG2200.023.4899928
GTTAGAC1253.5711673E-622.0325473
TCGACGT2209.094947E-1221.92993429
Produced by FastQC (version 0.11.5)