FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846759
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31260.169269514863607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25920.14035399313066838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.042.4534761
GTATCAA70600.040.521911
TATCAAC80300.035.3924522
ATCAACG84950.033.4720343
TCAACGC86300.032.84564
CAACGCA87550.032.56145
AACGCAG89650.031.951056
GTGGTAT11350.031.8483581
CGCAGAG97700.029.1778248
ACGCAGA98100.029.1288747
GCAGAGT102150.027.8394939
CAGAGTA101800.027.83324210
GCGCGTT500.00790640127.47711613
TGGTATC12900.027.4722162
AGAGTAC105550.026.81183811
AGTACGG64850.025.10438213
GAGTACG65600.024.92072912
AGTACTT49850.023.70142413
TACGGGG35350.022.93681315
GTACGGG74600.021.59661514
Produced by FastQC (version 0.11.5)