FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315440
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT14630.4637966015724068No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13010.4124397666751205No Hit
CTGTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC9470.3002155718995688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8120.2574182094851636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7660.2428354045143292No Hit
GGTCTATGGTGCCTCCCTATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA7600.24093329951813341No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7270.23047172203905655No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC7050.2234973370530053No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAAGCAATTCTTTAGCCTGGTATCAGCAGAAA6850.21715698706568604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6710.21271874207456248No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6610.2095485670809029No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT6500.20606137458787727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6030.1911615521176769No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA5900.18704032462591935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5750.1822850621354299No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCGTCACTT5580.17689576464620846No Hit
GTCTATTACTGTGCGAGGCAGTGGGAACTAAAGACATGGTTCGACCCCTGGGGCCAGGGAATCCTGGTCACCGT5560.17626172964747655No Hit
ACCTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAA5540.17562769464874461No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC5500.17435962465128077No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA5370.1702383971595232No Hit
ACCTACTACTACGCGTCCCTCAAGAGCCGGCTCACCATTTCCGTCCACGCATCTAGGAATCAGGTCTCCCTGAAA5340.1692873446614253No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5300.16801927466396147No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5210.16516611716966778No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA5160.16358102967283794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5100.16167892467664216No Hit
CCATAGACCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC4880.15470453969059092No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4770.1512173471975653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4740.15026629469946742No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4590.14551103220897796No Hit
CTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAAGC4480.14202383971595234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4430.1404387522191225No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4420.14012173471975653No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4410.13980471722039056No Hit
CTATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACACTCTCACCATCAGCAGC4390.13917068222165865No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4280.13568348972863303No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4240.13441541973116916No Hit
CAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT4150.13156226223687548No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4110.1302941922394116No Hit
TTCCAATAGGGAGGCACCATAGACCAGGAGACTAGGGGCTTTCCCTGGTT4090.12966015724067967No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4030.1277580522444839No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4010.12712401724575198No Hit
GATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTACC3950.12522191224955617No Hit
GTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCC3890.12331980725336039No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3760.11919857976160284No Hit
TTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCATCTGTCTT3740.11856454476287093No Hit
GGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTAC3670.11634542226730915No Hit
GTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCCCGATCCACTGCCA3660.1160284047679432No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3660.1160284047679432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3640.11539436976921126No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTAAGAGGGGTA3590.11380928227238142No Hit
CTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGA3560.11285822977428353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3500.11095612477808774No Hit
AGTCAGGGCATAAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAA3490.11063910727872178No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3470.11000507227998985No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3470.11000507227998985No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3380.10715191478569616No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3340.10588384478823232No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG3340.10588384478823232No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3260.10334770479330459No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3250.10303068729393863No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3220.10207963479584073No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC3180.10081156479837686No Hit
GGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3160.10017752979964495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)