FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469207
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12870.2742925830177299No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9850.2099286668783714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8780.1871242330144265No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACACCTTTTTAAATTGGTATCAGCAGAGA7950.16943481235360938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7680.1636804225000906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7550.16090979034839634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7180.15302414499357425No Hit
GAGTATTGTAAGCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT7040.1500403872917497No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6730.14343349523770957No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6670.14215474193692762No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6500.13853160758471209No Hit
ACAATACTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGA6490.13831848203458175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6330.13490847323249655No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTC6320.1346953476823662No Hit
GTATTGTAAGCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT6250.13320346883145393No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGGCTTACAATACTCCGTGGACGTT6020.12830158117845641No Hit
GGCTTACAATACTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT6020.12830158117845641No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6010.1280884556283261No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6010.1280884556283261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5840.12446532127611054No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC5670.120842186923895No Hit
GACTGGGGAAGGCCCCTAACCTCCTGATCTCTGGTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG5580.11892405697272206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5500.11721905257167946No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5470.11657967592128848No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5210.11103841161789998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5120.10912028166672706No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGGCACTGAACCTTG5070.10805465391607541No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4870.1037921429134689No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG4810.10251338961268694No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4810.10251338961268694No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC4730.10080838521164433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA150.002386837368.7643311
TTTACGC200.00735956451.7442333
GAATACG306.414856E-445.8428889
GTGGTAT1800.040.1810531
GTATCAA14700.040.0639081
CGAACTA350.001355550639.3778529
TATCAAC16150.036.4357152
TCAACGC17800.033.419944
ATCAACG17850.033.3369873
CAACGCA18100.033.2424285
AACGCAG18300.033.0670056
GTGTATA751.6888953E-632.144841
AGAGTAC19300.031.7099811
CGCAGAG19250.031.6137338
GTGCTAG553.2869814E-431.3099121
CCTATAC553.3117583E-431.269853
ACGCAGA19600.030.8737837
GCAGAGT20000.030.4282199
CAGAGTA20450.030.09490410
AATGCGG604.962702E-429.1837261
Produced by FastQC (version 0.11.5)