FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813631
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.192983026867097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.10680232086901911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.042.5022431
GTATCAA79400.041.2837261
GTGGTAT10250.036.8739621
TATCAAC89750.036.5611572
ATCAACG94950.034.6674233
TCAACGC96950.033.9522634
CAACGCA99250.033.23475
AACGCAG100300.033.058066
TCGTATG4150.030.85698540
ACGCAGA107800.030.723697
CGCAGAG110150.030.0041778
AGAGTAC113100.029.25195311
CAGAGTA114800.028.84870310
CTCGTAT4400.028.30222139
GCAGAGT117150.028.2113539
TGGTATC13700.028.089792
ACGAGAC7500.025.20346322
CTTATAC7900.024.791271
TCTCCGA7800.024.66519711
GAGTACT66700.024.41426512
Produced by FastQC (version 0.11.5)