FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438489
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11110.2533700959431137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA200.007509986451.47955711
GTGGTAT2850.041.041091
TGGTATC2850.041.022332
GGTATCA7550.040.553461
GTATCAA18150.039.8040731
GTCTAGG853.0431693E-936.4257431
TATCAAC21550.033.508762
TCAACGC22300.032.0148544
ATCAACG22700.031.4758473
CAACGCA22850.031.2406965
CTAGCTA450.004739532730.50640310
AACGCAG23800.030.2820936
ACGCAGA24350.029.1752727
CGCAGAG24600.029.018298
AGAGTAC25650.027.69660611
CAGAGTA25950.027.37641310
GCAGAGT26150.027.1670329
ATACTGT700.001373838724.5140766
GAGTACG19500.024.4637912
AGTACGG19900.024.31697813
Produced by FastQC (version 0.11.5)