FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798917
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35760.1987862697389596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30480.16943527689159643No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27890.15503772547593914No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27140.15086855035557506No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCTGCC26840.1492008803074294No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23370.12991149675054492No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGAGAGAGTGAAAT22380.1244081855916643No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACCCCACTCACTTTCGGCGGAGGGA22190.12335199456117209No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22070.12268492654191385No Hit
CTCCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACCCCACTCACTTT21940.12196226952105073No Hit
ACATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGA21640.12029459947290508No Hit
GAGTTACATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT21390.11890487443278373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21240.1180710394087109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19380.10773148511020797No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18810.10456291201873127No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18550.10311759797700505No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18400.10228376295293223No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18370.10211699594811767No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18270.10156110593206912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8750.048.7934151
ACGCGAT350.001081517541.23474567
GTATCAA61850.038.0214421
TATCAAC69450.033.837142
TCAACGC74000.031.624884
ATCAACG74950.031.424913
CAACGCA75600.030.988965
AACGCAG76050.030.8942176
AGAGTAC83200.028.61081311
CGCAGAG82800.028.583098
ACGCAGA84550.027.910237
GCAGAGT85850.027.647649
CAGAGTA88000.027.05022410
GGTATCA41150.026.9422341
AGGTACG658.796748E-426.4668242
TGGATTG16700.026.25861770
GAGTACT34750.024.51484912
AGTACGG63200.024.29600713
CGGGATA5250.024.22235117
GAGTACG63700.024.1046312
Produced by FastQC (version 0.11.5)