FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881315
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32370.3672920578907655No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22970.26063325825612865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22500.2553003182743968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21500.24395363746220136No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20100.2280682843251278No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18480.20968666140937123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17840.20242478568956615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17600.19970158229463925No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15830.17961795725705337No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15160.17201568111288246No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGCTTCAGCGGCAGTGGATCTGGGACACATTTCA15140.17178874749663856No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14700.16679620793927258No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14520.1647538053930774No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14380.16316527007937004No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13790.15647072840017473No Hit
GTCTCCATCCTCCCTGTCTGCATTTGTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAA13530.15352059138900392No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13490.15306672415651612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13460.15272632373215025No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13210.14988965352910139No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12880.1461452488610769No Hit
ATATAAAGACAGATGGAAGTGAGACATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG12800.14523751439610127No Hit
GTATGACAGTGTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12730.1444432467392476No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12670.1437624458905159No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCGCTTTATCTACAAATGAA12320.1397911076062475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12210.138542972716906No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12020.13638710336258886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11900.1350255016651254No Hit
CATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGCGACTTATTACTGTCAAAAGTATGACAGTGTC11880.1347985680488815No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11340.12867136041029598No Hit
CTGTCATACTTTTGACAGTAATAAGTCGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTC11290.1281040263696862No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11280.12799055956156424No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11170.12674242467222277No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11010.12492695574227149No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10770.12220375234734458No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10720.12163641830673481No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10520.11936708214429574No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10410.11811894725495425No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10220.11596307790063712No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10170.11539574386002735No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10130.11494187662753953No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9840.11165133919200285No Hit
AGTCAGGGCATTAGCAACTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCCAAGCTCCTGATCTATG9820.11142440557575894No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9800.11119747195951503No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA9750.11063013791890526No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9750.11063013791890526No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9360.10620493240214907No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG9330.1058645319777832No Hit
ACTTATTACTGTCAAAAGTATGACAGTGTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA9330.1058645319777832No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGGACA9290.10541066474529538No Hit
CTCCTGTGCAGCCTCTGGTTTTAGTTTTAGTAGTCATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAATGGG9240.10484333070468561No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9140.10370866262346606No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT9120.10348172900722216No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9070.1029143949666124No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9060.10280092815849043No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8990.10200666050163676No Hit
CTCCAGAGACAACGCCAAGAACTCGCTTTATCTACAAATGAACAGCCTGAGAGCCGAGGACACGGCCATCTATTT8900.10098545922853916No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8830.10019119157168549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3050.054.041051
CGCACGG400.00946396348.55417370
GTCTTTA1950.044.0238231
ATTGGCC1900.043.3652089
GTATCAA28900.043.2499771
ACGCTAA754.9740804E-543.15926770
TATTGGC2000.041.1969458
TATATTG2200.039.0211146
TATCAAC32850.037.8403852
CGTCAGA1250.035.7121245
ATATTGG2450.035.0314187
ATCAACG35750.034.8668673
TCAACGC36200.034.338584
CAACGCA36950.033.641595
CTTATAC1950.033.4581031
ACCCACT2500.032.9669116
TTATACA2100.032.7034152
AACGCAG38100.032.626166
ATGCCGT2150.032.2558744
CTTTATA2700.031.7949853
Produced by FastQC (version 0.11.5)