FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881315
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30340.3442582958420088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21630.24542870596778676No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20160.2287490851738595No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19780.22443734646522526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19540.22171414307029835No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19390.22001214094846902No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17540.1990207814459075No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16360.18563169808751695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15240.1729234155778581No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15200.17246954834537026No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15090.17122141345602876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15040.170654079415419No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGCTTCAGCGGCAGTGGATCTGGGACACATTTCA14660.16634234070678475No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14590.16554807304993108No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13790.15647072840017473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13180.14954925310473555No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.14830111821539405No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12390.14058537526310116No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12160.13797563867629623No Hit
CTGTCATACTTTTGACAGTAATAAGTCGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTC11980.13593323613010103No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11760.13343696635141805No Hit
GTCTCCATCCTCCCTGTCTGCATTTGTCGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAA11630.13196189784583265No Hit
ATATAAAGACAGATGGAAGTGAGACATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG11450.12991949529963748No Hit
GTATGACAGTGTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11360.12889829402653988No Hit
CATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGCGACTTATTACTGTCAAAAGTATGACAGTGTC11340.12867136041029598No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11320.12844442679405207No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11260.12776362594532034No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11220.12730975871283254No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG11050.1253808229747593No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10920.1239057544691739No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10730.12174988511485677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10400.11800548044683229No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10330.11721121278997862No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10270.11653041194124689No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10250.11630347832500298No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10200.1157361442843932No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10140.11505534343566148No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10080.11437454258692975No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10080.11437454258692975No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10030.11380720854631998No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCGCTTTATCTACAAATGAA9980.11323987450571021No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9910.11244560684885653No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9690.10994933707017356No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9610.10904160260519792No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9450.10722613367524665No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGCTT9370.10631839921027102No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGGACA9160.10393559623970998No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9130.10359519581534411No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9050.10268746135036848No Hit
GTAATAAGTCGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCACTGCCG8990.10200666050163676No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8920.10121239284478308No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8900.10098545922853916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT200.006988860752.42880254
GTGGTAT3050.049.6082881
GTATCAA28000.044.3353921
TTGCGTT350.001289006339.78693849
GTTGCGT350.001293028539.76173448
TATCAAC31700.039.0299072
ATCAACG33600.036.7893753
TCAACGC33450.036.6047944
CAACGCA34550.035.6217355
AACGCAG35300.034.66876
AGAGTAC37400.032.6285511
CGCAGAG37700.032.3707478
GTATTAC751.7117127E-632.0950621
CAGAGTA38050.031.98289310
GCAGAGT38800.031.1879639
ACGCAGA40750.030.0320267
TGTTAGA7150.028.72012770
GAGTACG25600.028.12052312
AGTACGG25600.027.98661613
GAGTACT18150.027.57530212
Produced by FastQC (version 0.11.5)