FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294011
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.14574837462741816No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC15850.12248736679981855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.042.560111
GTATCAA61300.039.1132931
GTGGTAT7750.036.344741
TATCAAC69750.034.4693342
TCAACGC72900.033.027034
ATCAACG72950.033.0043953
CAACGCA74250.032.4727975
ACGAGAC4000.031.77026222
CTCGTAT3050.031.76296439
AACGCAG77100.031.2724426
ACGCAGA82600.029.1856237
CGCAGAG83850.028.7914928
CGTATGC4000.028.59868241
AGAGTAC85750.028.11349911
TCGTATG3750.027.70287940
GCAGAGT87150.027.583079
CAGAGTA87200.027.56725310
TATGCCG4300.027.47066343
TGGTATC10150.026.3941442
TATACAC5650.026.1396063
Produced by FastQC (version 0.11.5)