FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294011
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40550.31336673335852633No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC15400.11900980749004451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61300.041.607771
GGTATCA24150.041.3639451
GTGGTAT7950.038.429871
TATCAAC70750.036.0349162
ATCAACG72000.035.33843
TCAACGC71900.035.2185944
CAACGCA73300.034.678135
TGGTATC9100.033.559062
AACGCAG76800.033.1423536
ACGCAGA81200.031.2620837
CGCAGAG81250.031.2428468
AGAGTAC84250.030.04785511
CAGAGTA85400.029.60426310
GCAGAGT85450.029.546859
GAGTACT48800.026.53552812
CGTCGAT659.0216805E-426.35192710
AGTACTT49400.025.86849813
GTACTTT52100.024.52885214
AGTACGG49900.024.5107713
GAGTACG50300.024.45018812
Produced by FastQC (version 0.11.5)