FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272773
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTGCTGCTATCATAAGACTGA67270.2959820448412578No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTGCTGCTATCATAAGACTGACAGTAGTAGT66190.2912301404495742No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT65320.2874022174673846No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61110.26887859016276594No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGCCAGCAACTATGTG55190.24283111423798154No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGCCAG54380.23926718594421878No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC52940.23293131342197396No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA48580.21374769939628813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG46340.20389189769501836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46190.2032319109739512No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44140.19421209245269985No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42380.18646824825884503No Hit
GACTACTACTGTCAGTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT41400.1821563350145395No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40210.17692044036073995No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG40120.17652444832809963No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39260.17274052446064786No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG39240.17265252623117222No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA38300.16851660944581795No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37400.1645566891194149No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC36950.1625767289562134No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36680.1613887528582925No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35730.1572088369582004No Hit
GTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC35370.15562486882763918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33820.1488050060432784No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33620.14792502374852218No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG33000.14519707863477788No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC32940.144933083946351No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGCCAGCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC32900.14475708748739974No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32240.1418531459147042No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31460.13842121496515492No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31090.13679324771985588No Hit
ATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30570.13450529375348969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.1334053158850444No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30040.13217334067238568No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29730.1308093681155135No Hit
CCCAATGATTGCTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC29730.1308093681155135No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29400.12935739732916574No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28990.12755343362491547No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC28900.12715744159227516No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAGG28390.12491348674064678No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCC26700.11747763634995664No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25840.11369371248250484No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA25790.1134737169088158No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA25250.11109776471297397No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT25060.11026178153295556No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24650.10845781782870528No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23880.1050698859938938No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTGCTGC23230.10220994353593606No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTG23140.10181395150329577No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23110.10168195415908232No Hit
CTACTACTGTCAGTCTTATGATAGCAGCAATCATTGGGTGTTCGGCGGAG23000.10119796389696639No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22990.10115396478222857No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22880.10066997452011266No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22830.1004499789464236No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22790.10027398248747235No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22740.10005398691378331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12850.045.729191
TGGTATC13500.044.26472
GGTATCA38750.043.0101131
GTATCAA103350.039.93311
TATCAAC110050.037.136332
ATCAACG118850.034.3684733
TCAACGC119400.033.9902084
CAACGCA121600.033.388775
AACGCAG123250.033.1072586
CGCAGAG133500.030.4368888
AGAGTAC135100.030.2794711
ACGCAGA135850.029.9608577
CAGAGTA136450.029.77885210
GAGTACT73150.026.34420812
AGTACTT73750.026.13160713
GAGTACG85050.025.3997512
AGTACGG84850.025.09756713
CGTAATT2951.209628E-924.97900470
GTACTTT77950.024.64051414
TACGGGG39750.023.03990215
Produced by FastQC (version 0.11.5)