FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776832
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13400.1724954687757456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11260.14494768495633548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10630.13683782336463998No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCTGCC10120.1302726973142198No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8110.10439837699785796No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7890.1015663618388532No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7840.10092272202998846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.046.7326131
GTATCAA30800.040.048811
TATCAAC35150.034.8807642
GAGGTAT1002.35263E-433.14499770
TCAACGC37300.032.4673924
ATCAACG37750.032.283433
GGTATCA17400.031.2870881
CAACGCA38800.031.2847635
AACGCAG39050.031.1725356
CGCAGAG43200.028.3371478
AGAGTAC43300.028.27170211
ACGCAGA43250.028.1453747
GCAGAGT44050.027.946479
GTACGCA500.007306829627.92269757
CAGAGTA44000.027.90007610
AACGAAC1101.0661206E-625.31480851
GAGTACG32650.025.0659212
AATAGCG851.300479E-424.72814862
AGTACGG32900.024.66641213
TTCGGGG4800.023.47770570