FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287477
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.17180889445015327No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC15280.11868173179016013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC200.00687110652.65598355
ATACGCG200.006904633352.59121354
GTGGTAT7950.041.8795851
GTATCAA59200.039.3682251
GGTATCA25050.037.6810261
TATCAAC66700.034.9929732
TGGTATC9750.033.443892
ATCAACG71200.032.8777543
TCAACGC71150.032.852624
CAACGCA73100.032.070165
CGCGTAT450.00456344130.74790239
AACGCAG76600.030.694436
ACGAGAC4300.030.33047322
CTTATAC4900.030.1210561
ACGCAGA82650.028.483597
CGCAGAG82850.028.4137278
CGAGACT4800.027.88607623
AGAGTAC84600.027.70428511
CAGAGTA85800.027.39680310
GCAGAGT87000.027.0189159
Produced by FastQC (version 0.11.5)