FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287477
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44420.3450158721282011No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC13860.10765240854788086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.10462322822077598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9400.043.4088061
TGGTATC10250.039.453022
GTATCAA63450.039.2341041
GGTATCA25750.036.0871
TATCAAC71300.035.1358072
TCAACGC73950.033.5966384
ATCAACG74500.033.374543
CAACGCA76700.032.5566185
AACGCAG79900.031.5095946
TAGCGGC1500.030.83623364
AATAGCG1301.4370016E-1030.00577562
CGCAGAG86400.028.9015338
ACGCAGA86900.028.8926947
AGAGTAC89150.027.89490111
CAGAGTA91000.027.44057710
AATACCG500.00806424127.3653955
GCAGAGT93350.026.713149
AACGAAC1508.6765795E-1025.70488451
CGAACCT1551.2405508E-924.92429753
GAGTACG47100.024.62014212
Produced by FastQC (version 0.11.5)