FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122446
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.12953852568408636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48600.041.4665571
GGTATCA19150.038.4309961
GTGGTAT6650.037.7518161
TATCAAC53800.037.5864832
CGCGGTA655.184502E-737.39235743
TCGTATG1950.037.29503640
ATCAACG55800.035.9311373
TCAACGC55950.035.8962754
CAACGCA57450.035.0188945
CTCGTAT1750.033.6205239
AACGCAG60750.033.2298556
TCTCGTA1750.031.61448938
TGGTATC8100.031.4183442
CGCAGAG64600.031.23838
CGTATGC2250.030.8191641
ACGCAGA65700.030.767617
TATGTCG450.00459975430.69779236
AGAGTAC67300.030.24046511
GACGGAC2650.029.85089126
CAGAGTA69100.029.50247610
Produced by FastQC (version 0.11.5)