FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122446
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27530.245267923802125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7750.042.5878371
GTATCAA50900.040.6625631
GGTATCA20850.037.5962751
TGGTATC8950.036.472422
TATCAAC56500.036.0636982
ATCAACG59300.034.2312243
TCAACGC59400.034.199384
CAACGCA60750.033.427485
AACGCAG63550.031.8992716
ATAAGCG450.004744851530.5035122
CGCAGAG68400.029.6374078
ACGCAGA68950.029.4507437
AGAGTAC71300.028.62439211
GCAGAGT72900.028.1373049
CAGAGTA73100.028.10724410
TAAGCGC658.9192286E-426.40315423
GAGTACG42300.025.38123912
AGTACGG42350.025.35353313
GAGTACT42150.023.030212
AGTACTT43100.022.68376413
Produced by FastQC (version 0.11.5)