FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220635
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.11141741798326281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53150.037.902081
GGTATCA25550.037.474991
GTGGTAT9100.033.941371
TATCAAC62350.032.637052
ATCAACG65300.031.110083
TCAACGC65750.030.9493544
CAACGCA67050.030.400475
AACGCAG69400.029.2721676
TGGTATC10400.028.376432
TCGTATG1850.028.067340
ACGCAGA74650.027.2561267
CGCAGAG75900.026.9428658
AGAGTAC77100.026.56802211
CAGAGTA78700.026.02788410
AGTACGG50950.025.92721213
GAGTACG50650.025.80980712
CTTATAC3900.025.518881
GCAGAGT80300.025.4665439
TACGGGG18550.024.41670615
TGTCGTA1502.8378054E-823.0375738
Produced by FastQC (version 0.11.5)