FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220635
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26360.21595317191461819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60350.040.3213461
GTGGTAT9750.039.7777251
GGTATCA24850.036.8766061
TGGTATC10700.036.861852
TATCAAC67200.035.98182
ATCAACG71350.033.766493
TCAACGC71600.033.4830324
CAACGCA72350.033.1291545
AACGCAG74300.032.2135936
ACGCAGA80400.029.769537
CGCAGAG81000.029.506748
AGAGTAC82450.029.11241111
CAGAGTA83950.028.51066210
GCAGAGT85350.028.0439
GAGTACG54100.026.5818412
AGTACGG54250.026.51051513
GTACGGG60250.023.30689414
TACGGGG21200.023.26494415
TACGGGC12200.022.45978215
GAGTACT43350.022.3527212
Produced by FastQC (version 0.11.5)