FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222028
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.12299227186283784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60000.038.7729721
GTGGTAT9350.038.1094171
GGTATCA26000.037.5562361
TATCAAC69000.033.9142462
CGTATGC1253.6379788E-1233.29200441
TGGTATC10750.031.8714712
ATCAACG73500.031.6980193
TCAACGC73300.031.6910254
CAACGCA74850.031.0805365
CGAGACG3450.030.77717223
AACGCAG78100.029.7433056
AGACGTA3700.029.62821225
TCGTATG1301.8735591E-1029.31355340
ACGAGAC3750.029.22958222
GAGACGT3750.028.31731824
ACGTAGA3900.028.12608727
ACGCAGA82750.028.0604427
CGCAGAG82900.027.9683578
CGTAGAG4000.027.4285528
AGAGTAC85600.027.16619511
Produced by FastQC (version 0.11.5)