FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222028
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29350.24017452955251434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59650.041.9182661
CGCGTAT350.001410847639.06384723
GTGGTAT7650.038.058211
GGTATCA22500.037.2970471
TATCAAC68100.036.6410982
TCAACGC70600.035.485164
ATCAACG70750.035.482933
CAACGCA72350.034.7014355
AACGCAG74350.034.0436336
ACGCAGA79450.031.8583287
CGCAGAG79650.031.692568
AGAGTAC81650.030.91625611
GCAGAGT83050.030.4773569
CAGAGTA83950.030.15061610
TGGTATC9800.028.6401862
GAGTACG52050.026.6442812
AGTACGG51650.026.45814513
ACGGGTA2900.025.92394416
GAGTACT45950.024.75468412
TACGGGG23050.023.86786715
Produced by FastQC (version 0.11.5)