FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300484
Sequences flagged as poor quality0
Sequence length21-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39880.30665506073123544No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA39610.3045789106209688No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35270.2712067199596458No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28520.21930296720297981No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28060.2157658225706737No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27620.21238246683542433No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT24750.19031376010777526No Hit
GTATATTACTGTGCGAAAGCATCGACCTCACTTTTAATCGCGGCGTACTACTTTGACTACTGGGGCCAGGGAACC23910.18385462643139017No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCAC21010.16155523635815588No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20940.16101697521845712No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20840.16024803073317317No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT20270.1558650471670547No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19310.1484831801083289No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18870.14509982437307956No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG18750.14417709099073883No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC18530.14248541312311416No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG18490.14217783532900058No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGT17840.13717969617465497No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG17660.13579559610114386No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC17650.1357187016526155No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17320.1331811848511785No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17010.13079745694679826No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16770.1289519901821168No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT16340.12564552889539587No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16320.12549173999833907No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16240.12487658441011193No Hit
CCCCTGTGCTGGTCATCTATCAAGATAACAAGCGGCCCTCAGGGATCCCT16210.12464590106452675No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16120.12395385102777119No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15670.12049360084399346No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15640.12026291749840827No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGCCGCGATTAAAAGTGAGGTCGATGCTTTCGCACAGTAATATA15590.11987844525576631No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14770.113573100476438No Hit
GTACTACCACTACTCCCACTAATAGATGCGACCCACTCCAGCCCCTTCTC14770.113573100476438No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC14530.11172763371175654No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14410.11080490032941581No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT14250.1095745891529615No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG14170.10895943356473436No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT14100.1084211724250356No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT13940.1071908612485813No Hit
AAGTAGTACGCCGCGATTAAAAGTGAGGTCGATGCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGG13940.1071908612485813No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC13760.1058067611750702No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC13670.10511471113831465No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA13530.10403818885891714No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13410.10311545547657643No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13060.10042414977808262No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG13010.10003967753544066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG200.007326861451.8077668
GTGGTAT2700.044.792811
GTATCAA17500.042.255161
TTGCGCG350.001339245639.4801729
ACGTATT350.001340512239.472588
GGATGGG14950.037.31054770
TATCAAC20650.035.288452
ATTTGCG501.8468834E-434.5464827
ATCAACG21800.033.2684823
TCAACGC21900.033.1165734
CAACGCA22200.032.6690525
AACGCAG22550.032.161996
CGCAAAC450.004599482830.69853617
GTGTATA1251.2369128E-1030.39505613
CGCAGAG24700.029.3647278
AGAGTAC24600.029.0640111
TAGAACA605.389729E-428.7798774
CAGAGTA24850.028.77161410
TCCGAGA16100.027.71640270
CTTATAC756.0982675E-527.6435621
Produced by FastQC (version 0.11.5)