FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300484
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG44860.34494849609837563No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA39630.30473269951802556No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35030.26936125319496435No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27300.20992184448251575No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT26270.20200171628409117No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26110.20077140510763686No Hit
GTATATTACTGTGCGAAAGCATCGACCTCACTTTTAATCGCGGCGTACTACTTTGACTACTGGGGCCAGGGAACC23680.1820860541152371No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC22610.17385834812269893No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22230.17093635907861998No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19410.14925212459361284No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19380.14902144124802766No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT19190.14756044672598817No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCAC18450.14187025753488702No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGATTACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG18020.13856379624816606No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG17760.1365645405864278No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG17370.13356565709382046No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT17190.13218155702030937No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17130.13172019032913898No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGT17120.1316432958806106No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16500.12687584007185018No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC16100.12380006213071441No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16050.12341558988807244No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16050.12341558988807244No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA15850.12187770091750456No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15640.12026291749840827No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15590.11987844525576631No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT15450.1188019229763688No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGCCGCGATTAAAAGTGAGGTCGATGCTTTCGCACAGTAATATA15400.11841745073372682No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15300.11764850624844288No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15230.11711024510874413No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC14940.1148803061014207No Hit
CCCCTGTGCTGGTCATCTATCAAGATAACAAGCGGCCCTCAGGGATCCCT14910.11464962275583553No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC14900.11457272830730714No Hit
AAGTAGTACGCCGCGATTAAAAGTGAGGTCGATGCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGG14480.11134316146911456No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14270.10972837805001831No Hit
GTACTACCACTACTCCCACTAATAGATGCGACCCACTCCAGCCCCTTCTC14220.10934390580737634No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT13790.10603744452065539No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13520.10396129441038873No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT13250.1018851443001221No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13130.1009624109177814No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT13080.10057793867513942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2800.044.3656961
GTATCAA20700.041.67471
GGATGGG17400.036.79841670
TATCAAC23700.036.2454642
ATCAACG24350.035.1362463
TCAACGC24650.034.568674
CAACGCA24950.034.2912865
AACGCAG25800.033.5678446
GTAGTTT1701.626031E-731.38688570
TCGTAAA553.257985E-431.3624482
CGCAGAG27800.031.0419068
AGAGTAC27600.030.76894811
GTCGTAA450.00461855430.6725791
CAGAGTA27750.030.60262710
GCAGAGT29050.029.4708169
TATAGGG605.423789E-428.748913
GTCTAGT500.00772800927.6053221
CGTAAAC658.6596276E-426.5374573
GAGTACG27350.026.00138512
AGTACGG27150.025.81147413
Produced by FastQC (version 0.11.5)