FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481720
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATGCTGCAACGTATTACTGTCATCAGAGTCGGAGTTTA33360.2251437518559512No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33110.22345652349971654No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT28060.1893745107037767No Hit
GTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGA23030.15542747617633562No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22590.15245795426936262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21790.14705882352941177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19720.13308857273978889No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18390.12411251788462056No Hit
ATTATAACCCGTCCCTCAAGAGTCGAGTTTCCATATCACGAGACACGTCC18060.12188537645439085No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18040.12175039818589208No Hit
GTGTAAGCTATTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT17820.12026563723240558No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17800.12013065896390682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.11979321329265988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17740.11972572415841048No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17740.11972572415841048No Hit
CAGTAATACGTTGCAGCATCTTCAGTTTCCAGCATATTGATGGTGAGGGTGAACTCTGTCCCAGATCCACTGCCA17400.11743109359393138No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16980.11459654995545716No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16050.10832006047026428No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16020.10811759306751613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.043.365891
GTATCAA25500.042.281751
GTGGTAT3100.039.016191
TATATAG459.961319E-538.369672
TATCAAC29250.036.952942
TCAACGC30250.035.9596754
ATCAACG30700.035.432583
CAACGCA31050.034.921965
AACGCAG32350.033.6253556
TGGTATC3450.033.0312842
TGTTAGA7150.031.70424370
AGAGTAC34400.031.0160611
CAGAGTA35250.030.56202110
AGTGCGT703.8295802E-529.59646410
ACGCAGA36850.029.5141547
CGCAGAG37000.029.301198
GCAGAGT38400.028.3237889
TCGTATG1004.855938E-727.70470840
GTCTTAA906.8195423E-626.8778211
AGTACGG36850.024.08224113
Produced by FastQC (version 0.11.5)