FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481720
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATGCTGCAACGTATTACTGTCATCAGAGTCGGAGTTTA32330.21819237102826444No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31670.21373808816780498No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT25830.17432443376616363No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20800.14037739923872256No Hit
GTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGA20360.13740787733174958No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19330.13045649650406282No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19320.13038900736981346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19160.12930918122182328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18910.12762195286558864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17910.12087303944065006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17480.11797100666792645No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC17450.1177685392651783No Hit
GGGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT17060.11513646302945225No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.11088464757174094No Hit
CAGTAATACGTTGCAGCATCTTCAGTTTCCAGCATATTGATGGTGAGGGTGAACTCTGTCCCAGATCCACTGCCA16110.10872499527576061No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15740.10622789730853333No Hit
GTGTAAGCTATTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT15530.10481062548929622No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15420.10406824501255298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.042.253211
GTATCAA27500.038.7314341
GGTATCA11650.036.3929251
TGGTATC5800.035.0557482
TATCAAC29950.034.9793662
ATCAACG31550.033.2054523
TCAACGC31850.033.0008854
CAACGCA32600.032.1359565
AACGCAG33650.031.4489216
AGAGTAC36450.029.24098611
CGCAGAG36700.028.944888
CAGAGTA37100.028.82067910
ACGCAGA37650.028.2088137
GCAGAGT39650.027.0541329
AGTACGG37250.023.98305913
GAGTACG38300.023.5957412
TAGTTCG800.00273281721.78401457
GGCTAGT800.002914632421.5435771
CGAAATT1506.883729E-720.83726748
TTCGGGG9500.020.63968770
Produced by FastQC (version 0.11.5)