FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317422
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30000.038.8213651
GGTATCA12650.037.1544231
GTGGTAT6150.033.1542361
ATCAACG35000.032.9567033
TCAACGC35300.032.676624
TATCAAC35400.032.5843122
TGGTATC6200.032.3073732
CAACGCA36350.031.8277345
AACGCAG37700.030.6880136
ACGCAGA39650.029.178777
AGAGTAC40950.028.3378711
CGCAGAG40850.028.3226958
CAGAGTA40950.028.2535310
GCAGAGT44500.026.232439
AGTACGG36900.024.52204913
GAGTACG39000.023.20163212
GGTCGAC952.8383965E-422.03939460
TACGGGG19650.021.79337515
GTACGGG42900.021.0923914
TACGGGT5000.020.03057115
Produced by FastQC (version 0.11.5)