FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317422
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.039.728951
GGTATCA13250.038.2497631
GTGGTAT5500.037.6110841
ATCAACG38100.034.744343
TATCAAC38100.034.47292
TCAACGC38500.034.1147424
CAACGCA38900.033.7639475
AACGCAG40100.032.7572826
ACGCAGA41900.031.444277
TGGTATC6550.031.0519262
CGCAGAG43100.030.5711148
AGAGTAC43500.030.13255711
CAGAGTA43950.029.74554810
GCAGAGT47400.027.5805249
AGTACGG39500.025.32459813
GAGTACG40400.024.76043512
GTATTAG4700.023.4735831
GTACGGG45550.021.50659214
GTACTTT15000.021.38618514
TACGGGG20350.021.18867115
Produced by FastQC (version 0.11.5)