FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395299
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC52350.3751884004790371No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43340.3106144274453002No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGG39930.28617522122498473No Hit
GCCCCCACACTCCTCATTTTTGACAATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT38740.2776465832771327No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32770.23486005508496746No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32670.23414336282044207No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31350.224683024928707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30830.22095622515317506No Hit
ACATATGATTCCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG29310.21006250273238927No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28660.2054040030129743No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27230.19515530363026132No Hit
GGTCAGAGGGTCACCGTCTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAA26490.18985178087277352No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25900.18562329651207377No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25170.18039144298103849No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24950.17881471999908263No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24560.17601962016743364No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGACTACTATTG24160.17315285110933212No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA23170.16605759769053086No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22740.1629758209530717No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21980.1575289597426788No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21710.1555938906284603No Hit
ATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGACATCA21210.15201042930583336No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21200.15193876007938084No Hit
CCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTGCTCTGG21040.15079205245614022No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21010.1505770447767826No Hit
ATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGG20760.14878531411546916No Hit
GTGTGGGGGCTGCTCCCGGGAGTTGTTGGTACCAGGATACATAATTATTG20680.14821196030384887No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG20250.1451301835663897No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20100.14405514516960166No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19860.1423350837347407No Hit
ATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG19740.14147505301731028No Hit
CCGCAGCACTCAGGCTGGAATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGT19510.13982666080890188No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19430.13925330699728158No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19360.1387516224121138No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19070.1366732148449902No Hit
GTCCTGGGCCCAGTCTGTTTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTG19020.13631486871272752No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18870.13523983031593945No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18410.1319430458991227No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT18070.1295062921997364No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17870.12807290767068563No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC17810.12764289231197043No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17540.12570782319775187No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGGC17220.12341440795127068No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16900.12112099270478943No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA16880.12097765425188438No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16820.12054763889316913No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16120.11553079304149146No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC16080.11524411613568131No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAG16050.11502910845632369No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15990.11459909309760848No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15860.11366739315372548No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15700.11252068553048486No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15620.11194733171886455No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15500.1110873010014341No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15360.11008393183109856No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTCTCCTGTG15060.10793385503752241No Hit
CTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCC14940.10707382432009196No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG14900.1067871474142818No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14700.10535376288523104No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14580.1044937321678006No Hit
TCACCGGACTCCAGACTGGGGACGAGGCCGACTACTATTGCGCAACATAT14300.10248699382712952No Hit
GACTACTATTGCGCAACATATGATTCCAGCCTGAGTGCTGCGGTGTTCGG14230.10198530924196175No Hit
GCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCCCACA14150.10141195543034145No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14120.10119694775098384No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13970.10012190935419576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)