FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395299
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTTTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTG41080.2944171822670266No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40010.28674857503660506No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC38400.2752098295777464No Hit
GCCCCCACACTCCTCATTTTTGACAATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT35950.25765086909687457No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGG32520.233068324423654No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32090.22998654768619486No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28820.20655071063621488No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27920.20010048025548643No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26740.19164351153408696No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26330.1887050732495329No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26020.18648332722950423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25970.18612498109724152No Hit
ACATATGATTCCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG25940.18590997341788393No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24750.17738133547003188No Hit
GGTCAGAGGGTCACCGTCTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAA24660.17673631243195903No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23270.16677428995505622No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23010.16491089006729023No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGACTACTATTG22860.16383585167050216No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22200.15910568272463466No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20520.14706525268060824No Hit
ATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGACATCA20490.14685024500125063No Hit
CCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTGCTCTGG20480.1467785757747981No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG20360.14591854505736762No Hit
GTGTGGGGGCTGCTCCCGGGAGTTGTTGGTACCAGGATACATAATTATTG20330.14570353737801No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19960.1430517759992661No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT19650.1408300299792374No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19640.1407583607527849No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA19630.14068669152633237No Hit
GCACAGGGTCCTGGGCCCAGTCTGTTTTGACGCAGCCGCCCTCAGTGTCT19210.13767658401532576No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18950.13581318412755977No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18780.1345948072778666No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18770.13452313805141408No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18470.13237306125783793No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18410.1319430458991227No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18360.13158469976686002No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17940.1285745922558534No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17700.1268545308209925No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17660.12656785391518233No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17420.12484779248032142No Hit
GGGTCCTGGGCCCAGTCTGTTTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTC17420.12484779248032142No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC17130.12276938491319782No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG17070.12233936955448259No Hit
GTTTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCAC16960.12155100806350468No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA16960.12155100806350468No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16500.11825422364668793No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTCTCCTGTG16460.11796754674087775No Hit
ATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGG16460.11796754674087775No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC16310.1168925083440897No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15820.11338071624791533No Hit
GTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC15510.11115897022788664No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15430.11058561641626634No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15210.1090088934343105No Hit
GCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCCCACA14650.10499541675296836No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14620.10478040907361075No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT14380.10306034763874984No Hit
CTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCC14370.1029886784122973No Hit
ATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG14310.10255866305358206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14310.10255866305358206No Hit
CCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGA14240.1020569784684143No Hit
GACTACTATTGCGCAACATATGATTCCAGCCTGAGTGCTGCGGTGTTCGG13960.10005024012774322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)