FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238139
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC38650.31216204319547325No Hit
ACATATGATACCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG33550.2709711914413487No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32330.26111769357075415No Hit
GCCCCCACACTCCTCATTTTTGACAATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT32160.25974466517895No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGG31920.25780627215522656No Hit
ATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGG28200.22776118028751216No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28070.2267112173996619No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27710.22380362786407665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27310.22057297282453747No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26850.21685771952906743No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26170.21136560596185083No Hit
GTGTGGGGGCTGCTCCCGGGAGTTGTTGGTACCAGGATACATAATTATTG26030.21023487669801208No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24030.1940816015003162No Hit
GGTCAGAGGGTCACCGTCTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAA23930.19327393774043142No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23880.19287010586048903No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23780.19206244210060422No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA23520.18996251632490374No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23430.18923561894100743No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23420.18915485256501893No Hit
ATATGATACCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC22580.18237047698198666No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21490.1735669419992424No Hit
ATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGACATCA21140.17074011883964563No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21040.16993245507976085No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGACTACTATTG20980.16944785682382996No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20490.16549030440039447No Hit
GTATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTG20390.16468264064050966No Hit
ATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG19900.16072508821707418No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19550.1578982650574774No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG19110.1543445445139843No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19090.15418301176200733No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19050.1538599462580534No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC18970.15321381525014557No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18670.1507908239704912No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17900.14457181301937827No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17820.14392568201147043No Hit
CCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTGCTCTGG17760.14344108375553957No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17730.14319878462757413No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17640.14247188724367782No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTCTCCTGTG17510.14142192435582757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17450.1409373260998967No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17300.1397258304600695No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17150.1385143348202423No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17040.13762590468436905No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16840.13601057716459944No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15990.1291454352055787No Hit
CCTTGGGAGAATCCCCTAGATTACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG15630.12623784566999344No Hit
CCGCAGCACTCAGGCTGGTATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGT15620.12615707929400496No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15250.1231687233824312No Hit
ATACCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT15020.12131109673469617No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGGC14860.1200188347188805No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA14750.11913040458300724No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14710.11880733907905332No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14690.11864580632707637No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAG14680.11856503995108789No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14510.11719201155928373No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14380.11614204867143349No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14350.11589974954346806No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14260.11517285215957174No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14140.11420365564771No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA13510.10911537396043579No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13480.10887307483247034No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13450.10863077570450491No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13420.10838847657653947No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC13390.10814617744857402No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13340.10774234556863163No Hit
GCACTAACAGTGGGAGCACAAACTATGCACAGAAGGTCCGGGGCAGAGTC13330.10766157919264314No Hit
CTCCCAGCTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTCTCCTGTGAGG13220.10677314905676988No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13030.10523858791298876No Hit
CTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCC13000.10499628878502332No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12970.1047539896570579No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12850.10378479314519613No Hit
GCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGAGCAGCCCCCACA12820.1035424940172307No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG12800.10338096126525373No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12770.10313866213728831No Hit
CCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAACTCCCGGGA12700.10257329750536893No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12560.10144256824153022No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12520.10111950273757632No Hit
GTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC12510.10103873636158782No Hit
GACTACTATTGCGCAACATATGATACCAGCCTGAGTGCTGCGGTGTTCGG12420.10031183897769153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)