FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028963
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10960.10651500588456533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.041.172391
GTATCAA20800.037.9731641
TATCAAC23750.033.2354852
ATCAACG24450.032.424943
TCAACGC24450.032.424944
CAACGCA25200.031.5966935
AACGCAG25750.030.7879526
AGAGTAC27850.028.71674211
CGATTGT900.007035899428.13836370
CGCAGAG28950.027.5051948
CAGAGTA29050.027.41184410
ACGCAGA29700.026.8093157
GCAGAGT29950.026.5881169
AGTACGG27800.024.05632813
GAGTACG28200.023.71510312
TGTTAGA2553.5064477E-722.34517170
CGTTTTA953.0758354E-421.77843725
TACGGGG16450.021.58353615
GTACGGG31350.021.22228614
TCGATTG1000.003744561520.63114569
Produced by FastQC (version 0.11.5)