FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821239
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC107150.5883357428651594No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACGCTGGCTGCTGTCATAGGACTGG105960.5818017294819625No Hit
CTCCTGCACTGCGAGCAGCCCCAACTTCGGGGCAGGTCATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC92590.508390167353104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65970.36222593520125584No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG62370.34245917202519827No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC61630.3383960040390086No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57900.3179154410815933No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC55630.30545139874557925No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG54100.2970505243957548No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT53770.29523857110461615No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT49400.2712439169159018No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC48310.2652589802875954No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG47430.2604271048445591No Hit
GTCCTATGACAGCAGCCAGCGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGC46630.2560344908054352No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCAT45520.24993973882615075No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG43330.23791495789404907No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA42640.23412632828530466No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC42530.23352234385492512No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATCGTGACTACAATCGGCCCTCAGAGGTCCCTGCCCGATTCTCTGG41340.22698833047172828No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC40930.2247371157766773No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40400.22182700897575772No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG40120.22028959406206433No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT39910.21913653287679433No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCAGCGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT39270.21562244164549516No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACGCTGGCTGCTGTCATAGGACTGGCAGTAATAAT38850.21331631927495512No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38290.2102414894475684No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA38060.20897861291132025No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37930.20826481312996264No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37410.2054096140045321No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT37260.20458599887219633No Hit
GTCTGGCACCTCAGCCTCCCTGACCATCACTGGGCTCCAGGCTGAGGATG35350.194098632853788No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33720.185148681749073No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGCGAGCAGCCCCAACTTCGGGGCAGGTCATGATGTACACTGGTACCAG33500.18394071288831393No Hit
GTGATGGTCAGGGAGGCTGAGGTGCCAGACCGGGAGCCAGAGAATCGGGC32660.17932846814723385No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACGCTGGCTGC32380.17779105323354047No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT31900.1751554848100661No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31600.17350825454539465No Hit
ATATTACACGCTGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCA31220.17142176287681077No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGTCAGGGAGG30420.16702914883768685No Hit
GGTCATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATCGTGACTACAATCGG30370.16675461046024165No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30250.16609571835437303No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC30240.166040810678884No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29120.15989115102411053No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC28650.1573104902761252No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT28510.15654178281927852No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGCGAGCAG28260.1551690909320523No Hit
GCCCCAACTTCGGGGCAGGTCATGATGTACACTGGTACCAGCACCTTCCA28040.15396112207129323No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG27790.152588430184067No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC27370.15028230781352694No Hit
GACCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT27290.14984304640961454No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC27260.1496783233831474No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC27200.14934887733021313No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26240.1440777404832644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26050.14303449464897247No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCAGCGTGTAATATTCGGCGGAGGGACCAAGCTGAC25430.13963021876865145No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATGACCTGCCCCGAAGTT25200.13836734223240332No Hit
GTGCCAGACCGGGAGCCAGAGAATCGGGCAGGGACCTCTGAGGGCCGATT25110.13787317315300188No Hit
GCCTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCCAGACCGGGAGCC24810.1362259428883304No Hit
GTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC24750.13589649683539612No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC24740.13584158915990707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG24560.1348532510011042No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24530.13468852797463704No Hit
GCCCAGTGATGGTCAGGGAGGCTGAGGTGCCAGACCGGGAGCCAGAGAAT24460.13430417424621371No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24320.13353546678936704No Hit
GCTTGGTCCCTCCGCCGAATATTACACGCTGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAG24170.13271185165703128No Hit
GCCCCCAAACTCCTCATCTATCGTGACTACAATCGGCCCTCAGAGGTCCCTGCCCGATTCTCTGGCTCCCGGT24170.13271185165703128No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC23690.13007628323355694No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23560.12936248345219928No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG23480.1289232220482869No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23440.12870359134633072No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCC23280.12782506853850595No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23210.1274407148100826No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22990.12623274594932352No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACGCTGG22890.12568366919443302No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCA22880.12562876151894398No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG22400.12299319309546962No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC22360.12277356239351342No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT22070.12118123980433101No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT21690.11909474813574714No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATCGTGACTACAATCGGCCCTCAGAGGTCCCTGC21650.11887511743379096No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC21530.11821622532792236No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC21490.11799659462596616No Hit
GTCATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATCGTGACTACAATCGGC21430.11766714857303189No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21410.11755733322205378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21390.11744751787107569No Hit
GTCCTGGGCCCAGTCTATATTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG21340.11717297949363044No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA21300.11695334879167424No Hit
GGGTCACCATCTCCTGCACTGCGAGCAGCCCCAACTTCGGGGCAGGTCATGATGTACACTGGTACCAGCACCTTC21140.11607482598384945No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGCGAGCAGCC21120.11596501063287136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20660.1134392575603751No Hit
TCCTCAGCCTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCCAGACCGGGAGCCAGAGAATCGGGCAGGGAC20640.113329442209397No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20030.10998007400456501No Hit
CTTCGGGGCAGGTCATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATCGTGA19830.10888192049478404No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGCGAGCAGCCCCAACTTCGGGGCAGGT19420.10663070579973304No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19320.10608162904484256No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19310.1060267213693535No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC18970.10415986040272585No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG18960.1041049527272368No Hit
GTGTACATCATGACCTGCCCCGAAGTTGGGGCTGCTCGCAGTGCAGGAGA18870.10361078364783535No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18530.10174392268120769No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18500.10157919965474053No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18230.10009669241653621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.047.3452031
GTATCAA39150.044.870831
TCGTATG1950.042.55516440
TATCAAC43750.040.5143622
TCAACGC46800.037.725884
ATCAACG46950.037.679693
CGCGTAG2200.037.5783928
CAACGCA47650.037.052915
ACGCGTA2150.036.84607327
GCGTAGT2250.036.74735329
AACGCAG48550.036.295156
CGTATGC2250.035.3649141
AGACGCG2250.035.19783425
ACGCAGA50100.035.1035547
CGCAGAG51350.034.2490358
TGCCGTC2250.033.9002845
TATGCCG2350.033.89650343
CGAGACG2350.033.69172323
AGAGTAC52800.033.3773311
TGGTATC3200.033.341992
Produced by FastQC (version 0.11.5)