FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838599
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11410.1360602624138593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.041.2234541
GGTATCA11200.038.0555271
CGAATTA754.1383828E-836.67737215
TCAACGC32900.035.7245334
ATCAACG33000.035.616283
TATCAAC33200.035.6087532
CAACGCA33300.035.4007265
AACGCAG34200.034.772686
ACGCAGA35600.033.314637
AGAGTAC36150.032.7185311
CGCAGAG36800.032.0471848
CAGAGTA37500.031.4489710
AACCGTA450.004401383530.97471449
GCAGAGT38300.030.7023039
CTCGCTA703.9352548E-529.47640623
GTGGTAT4200.029.4623411
AGTACGG30600.028.7648513
GAGTACG30700.028.67115412
AACGCTA657.732845E-427.05531361
ACGGGAT2850.026.53808816
Produced by FastQC (version 0.11.5)