FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579575
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7350.1268170642280982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.044.864341
GGTATCA6900.038.3774381
TCAACGC23600.037.5668454
CAACGCA23600.037.4212385
ATCAACG23650.037.3421253
TATCAAC23700.037.263342
AACGCAG23900.036.9547046
ACGCAGA24700.035.7670487
AGAGTAC25350.034.84994511
CAGAGTA26150.033.7837910
CGCAGAG26250.033.655098
GCAGAGT26850.032.903029
AGTACGG21550.030.7862613
GAGTACG21550.030.62674712
CGCCGTA450.004679587730.5875325
TACGGGG9500.028.9476515
AAATCGT500.007566084627.72277641
GTACGGG23700.027.70332714
ACCGGTG500.007685706527.6338236
AATAGCG1554.0017767E-1127.24087562
Produced by FastQC (version 0.11.5)