FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005491
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC82910.4134149692020557No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAGCAC67340.33577812116833233No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC65240.3253068699884467No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT64420.32121809571820564No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG59600.2971840811053253No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC56490.2816766567389233No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAGCACACCACTCAGG55720.27783719797296524No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53430.26641854787680425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51780.2581911362354655No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA51080.25470071917550363No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCGCTGATGGCCAGGGAGG48840.24353138458362567No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48490.24178617605364472No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC48000.2393428841116714No Hit
GCATGGGATGACACCCTGAGTGGTGTGCTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG47990.23929302101081482No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC47870.23869466380053564No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG47010.23440643712686818No Hit
GATTATTACTGCGCTGCATGGGATGACACCCTGAGTGGTGTGCTTTTCGG46720.23296040720202685No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC46240.23056697836091014No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG46210.2304173890583403No Hit
GGATGACACCCTGAGTGGTGTGCTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC46130.23001848425148755No Hit
GTGTCATCCCATGCAGCGCAGTAATAATCAGCCTCATCCTCGGACCGGAG45530.22702669820009164No Hit
ATCCCATGCAGCGCAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCGC44210.2204447688870207No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43770.21825079244933038No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40270.200798707149521No Hit
CTACAGGACTAATCAGCGGCCCTCAGGAGTCCCTGACCGCTTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCC38120.19007814046535237No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38050.18972909875935617No Hit
GGACTAATCAGCGGCCCTCAGGAGTCCCTGACCGCTTCTCTGCCTCCAAG37050.18474278867369637No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG36410.1815515502188741No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35650.1777619545537726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35250.1757674305195087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG34540.17222715035869024No Hit
CCTCAGGAGTCCCTGACCGCTTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCGGT34540.17222715035869024No Hit
GGTCCAACCTCGGTCGTAATTTTGTATACTGGTTCCAGCAGCTCCCAGGA34130.17018276322356968No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34050.1697838584167169No Hit
CTCCTGAGGGCCGCTGATTAGTCCTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCAGTATA32500.16205507778394418No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32370.16140685747280842No Hit
GTCCTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGAACCA32090.16001069064882364No Hit
GATTAGTCCTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG31570.15741780940428055No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31490.15701890459742776No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30540.15228191001605093No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30240.150786016990353No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAGCACACCACTCAGGG29750.14834272504837967No Hit
CTCTTGTTCTGGAAGCAGGTCCAACCTCGGTCGTAATTTTGTATACTGGTTCCAGCAGCTCCCAGGAACGGCCCC29330.14624847481240255No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29010.1446528555849914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28710.14315696255929347No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC28520.1422095636430181No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28220.14071367061732015No Hit
GTCCCTGACCGCTTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGC28040.1398161348019014No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC27670.13797120007020724No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT27660.13792133696935066No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27420.1367246225487923No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG27350.1363755808427961No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCGGTCCGAGGATG27050.13487968781709817No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG27050.13487968781709817No Hit
GTCCAACCTCGGTCGTAATTTTGTATACTGGTTCCAGCAGCTCCCAGGAA27040.13482982471624158No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG26850.1338824257999662No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG26660.13293502688369083No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26570.13248625897598143No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26400.13163858626141928No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25890.12909556811773276No Hit
GCCCTCAGGAGTCCCTGACCGCTTCTCTGCCTCCAAGTCTGGCACCTCAG25790.12859693710916678No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25580.12754981199117824No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAGCACACCACTCAGGGTGTCATCCCATGCAGCGCAGTAAT25340.12635309757061985No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACCTCGGTCGTAATTTT25190.12560515105777087No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAGCACAC24820.12376021632607676No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTACAGGACTAATCAGCGGCCCTCAGGAGTCCCTGACCGCTTCTCTGC24260.12096788267810725No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23840.11887363244213013No Hit
GGGTGTCATCCCATGCAGCGCAGTAATAATCAGCCTCATCCTCGGACCGG23770.11852459073613394No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACCTCGGTCGTAATTTTGTATA23640.11787637042499817No Hit
CCTTGGTCCCTCCGCCGAAAAGCACACCACTCAGGGTGTCATCCCATGCAGCGCAGTAATAATCAGCCTCATCCT23610.11772678112242838No Hit
CCACTCAGGGTGTCATCCCATGCAGCGCAGTAATAATCAGCCTCATCCTC23600.11767691802157178No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23430.1168292453070096No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23350.11643034050015683No Hit
GTCCGAGGATGAGGCTGATTATTACTGCGCTGCATGGGATGACACCCTGAGTGGTGTGCTTTTCGGCGGAGGGAC23150.11543307848302486No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23070.11503417367617207No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22870.11403691165904009No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC22680.11308951274276474No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG22460.11199252452391958No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22200.11069608390164803No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21840.10890101227081048No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC21690.10815306575796152No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21640.10790375025367853No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG21570.10755470854768234No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21530.10735525614425595No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAAG21500.10720566684168614No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21440.10690648823654657No Hit
GTGCCAGACTTGGAGGCAGAGAAGCGGTCAGGGACTCCTGAGGGCCGCTG20770.10356566047915448No Hit
GTCAGGGACTCCTGAGGGCCGCTGATTAGTCCTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGAA20280.10112236853718118No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20250.10097277923461138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)