FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331478
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34040.2556557449691245No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32280.24243735157471621No Hit
GTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC30960.23252355652891No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28890.21697692338889565No Hit
GTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGTCA27760.2084901139936221No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27440.20608676974009335No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26040.19557213863090492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25930.1947459890437544No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25110.18858741939408685No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCA24590.18468198498210261No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22990.17266526371445867No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22880.17183911412730815No Hit
CTACAAGCCGTCCCTCCAGAGTCGAGTCACCATGGCCGTGGACACGTCCAAGAAGGAACTCTCCCTGAAGTTGAA22400.16823409774701498No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22390.1681589932390922No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22320.16763326168363277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21880.1643286633350307No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGAGAGTTCCTTCTTGGACGTGTCCACGGC21430.16094896047850585No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21380.16057343793889198No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21250.1595970793358959No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG21180.15907134778043647No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21000.15771946663782654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20760.15591695844767994No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20270.152236837559464No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC20060.15065964289308573No Hit
GTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT19790.14863182117917081No Hit
GTCTGGGACCCCAGAGTCCCGGTGAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA19220.14435086422757268No Hit
CACTTGTACTGTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGG19070.14322429660873104No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19010.14277366956119442No Hit
CTATAATACATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGAATCCAGACCCAGTAATAACTGTTC18960.14239814702158052No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA18740.1407458478472795No Hit
GGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGA18460.1386429216254418No Hit
GTGTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGC18230.136915517943218No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG18040.13548853229268526No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17940.13473748721345752No Hit
GTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCATA17930.13466238270553474No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17820.13383623311838425No Hit
CTAGTACACTGAACGCCGGAAAAACTCGCACAATAATACACAGCCGTGTC17770.13346071057877035No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17610.13225903845200596No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17530.13165820238862377No Hit
GTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGCCAGGGAATCCTGGTCATCGTCTCCTCAGCCTC16980.12752745445287117No Hit
ATGTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGT16790.12610046880233847No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGT16750.12580005077064738No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16600.12467348315180574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16230.12189461635866308No Hit
GTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGGTCTGGATTCG16220.12181951185074032No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16150.12129378029528089No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16130.12114357127943534No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15640.11746345039121937No Hit
GCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGGCCAGTATGAACCTTGCATG15550.11678750981991443No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15530.11663730080406887No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15210.11423395655054008No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15020.11280697090000737No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGA14950.11228123934454795No Hit
GTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT14860.11160529877324296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14710.11047873115440135No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14580.10950237255140528No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14490.10882643198010032No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14280.10724923731372206No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA14180.1064981922344943No Hit
GTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGCCAGGGAATCCTGGTCATCGTCTCCTCAGCCTCCA14170.10642308772657151No Hit
GTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCT14080.10574714715526655No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGG14070.10567204264734378No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACT13880.10424505699681105No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13730.10311848937796944No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13690.10281807134627835No Hit
GTTCTAGTACACTGAACGCCGGAAAAACTCGCACAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAA13620.10229233979081892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13610.10221723528289615No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACTCTCC13600.10214213077497337No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT13580.10199192175912783No Hit
GATGGGTCCTGTCCCAATTGCAGCTGCGGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACTCTGTCCCTCA13570.10191681725120504No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13530.10161639921951394No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13470.10116577217197731No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13420.10079024963236342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)